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Essential4Health

Get Healthy ~ Stay Healthy

Essential4Health

Get Healthy ~ Stay Healthy

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Reverse Transcription Polymerase Chain Reaction

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RT-PCR

Description

"Reverse Transcription Polymerase Chain Reaction" (RT-PCR) test detects RNA-based viruses by converting RNA into DNA before amplification. It’s frequently used to diagnose COVID-19, influenza, or hepatitis C when symptoms like cough, fatigue, or fever are present. A sample—typically from the nose, throat, or blood—is analyzed for viral RNA. Positive results confirm infection and guide antiviral treatment. RT-PCR offers high sensitivity and is especially valuable for early detection, outbreak control, and monitoring response to therapy.

"Reverse Transcription Polymerase Chain Reaction" (RT-PCR) test detects RNA-based viruses by converting RNA into DNA before amplification. It’s frequently used to diagnose COVID-19, influenza, or hepatitis C when symptoms like cough, fatigue, or fever are present. A sample—typically from the nose, throat, or blood—is analyzed for viral RNA. Positive results confirm infection and guide antiviral treatment. RT-PCR offers high sensitivity and is especially valuable for early detection, outbreak control, and monitoring response to therapy.

Category

Microbes

Procedure

Non-Invasive

Sample Type

Saliva | Fluid

Units

Not Applicable

Procedure Category

Swab, Measure

Test Group

Microbial Assessment Group, Infection Group

Test Group Description

Microbial Assessment Group: Tests in this group analyze microbial presence and activity in various samples, offering insights into microbial populations and their impact on health. They help identify specific microorganisms, assess microbial diversity, and evaluate microbial functions in different environments. Infection Group: Tests within this group detect markers indicative of various infections, aiding in the diagnosis and management of infectious diseases.

Optimal Range

For All Individuals:

  • Result: Negative: No abnormalities detected.

Normal Range

For All Individuals:

  • Result: Negative: No abnormalities detected.

Results That Differ From The Norm (Direct and Indirect Causes)

Abnormal results may indicate:


  • Autoimmune disease markers

  • Bacterial RNA detection

  • Cancer markers

  • Genetic mutations

  • Human immunodeficiency virus (Detection of HIV RNA indicating HIV infection)

  • Influenza viruses

  • Respiratory syncytial virus (Common virus causing respiratory tract infections, especially in young children)

  • SARS-CoV-2 (Detection of RNA from SARS-CoV-2 virus, indicating COVID-19 infection)

Key Reasons For Testing

  • Viral RNA Detection: Amplifies viral RNA to diagnose infections such as COVID-19, influenza, and HIV with high sensitivity.

  • Early Diagnosis of Infectious Diseases: Enables timely treatment and infection control, preventing disease transmission.

  • Screening of Asymptomatic Carriers: Detects infections in asymptomatic individuals to reduce unintentional spread.

  • Genetic Variant Identification: Identifies viral variants, aiding in tracking viral evolution and informing treatment and vaccine strategies.

Health Status Conditions It May Be Used To Assess

Currently, this test is not directly associated with any conditions listed on the Health Status page. However, it may be included as part of a broader set of tests linked to specific health conditions.

Some Prominent Medical Labs That May Offer This Test

Please note that this particular test has not been associated with any of the listed prominent medical labs. We recommend enquiring with your private physician or nearest hospital to determine where this specific test can be performed.

References

Important Note

Any medical procedure yielding results outside the norm may be directly or indirectly linked to the conditions outlined on this page. Various factors, including genetics, medication and supplement usage, recent illnesses, pregnancy, pre-test eating, smoking, and stress, can impact the test's outcome. Additionally, factors like false positives, false negatives, inaccurate analyses, and others can influence results.


Reference ranges, which help healthcare professionals interpret medical tests, may vary depending on age, gender, and other factors. They may also differ between laboratories due to variations in instruments and methods used. Optimal ranges are designed for preventive purposes, aiming to identify trends and potential risks early, while normal ranges reflect conventional laboratory values indicating no current disease or pathology. Your healthcare practitioner may have specific reasons for testing that deviate from the usual or may interpret results differently based on individual circumstances. Proper interpretation typically involves considering clinical findings and other diagnostic tests. Hence, it is crucial to provide your healthcare professionals with a comprehensive medical history, consult with them for result interpretation, and follow their guidance for potential re-testing or additional diagnostics.

Disclaimer

This content is provided solely for informative and educational purposes. It is not intended as a substitute for medical advice or treatment from a personal physician. Regarding the interpretation of their medical test results and/or specific health questions, it is recommended that all readers and viewers consult their physicians or other qualified health professionals. The publisher is not responsible for any adverse health effects that may result from reading or following the information in this educational content. Before beginning any nutrition, supplement, or lifestyle program, all viewers, especially those taking prescription or over-the-counter medications, should consult their physician or health care practitioner.


Please note that while prominent lab names are included in this content, we cannot guarantee that these labs offer all the tests mentioned. For confirmation, individuals should contact the labs directly or consult their medical practitioners. The information provided reflects general knowledge at the time of publication and may not include recent updates or emerging research. Readers should verify details with qualified professionals to ensure the most up-to-date and accurate guidance.

[1] Heid CA, et al. Real-time quantitative PCR. Genome Res. 1996;6(10):986-994.

[2] Nolan T, Hands RE, Bustin SA. Quantification of mRNA using real-time RT-PCR. Nat Protoc. 2006;1(3):1559-1582.

[3] Wong ML, Medrano JF. Real-time PCR for mRNA quantitation. Biotechniques. 2005;39(1):75-85.

[4] Kubista M, et al. The real-time polymerase chain reaction. Mol Aspects Med. 2006;27(2-3):95-125.

[5] Ginzinger DG. Gene quantification using real-time quantitative PCR: an emerging technology. Genes Chromosomes Cancer. 2002;34(2):199-207.

[6] Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(−ΔΔC(T)) method. Methods. 2001;25(4):402-408.

[7] Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001;29(9).

[8] Bustin SA. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol. 2000;25(2):169-193.

[9] Higuchi R, et al. Simultaneous amplification and detection of specific DNA sequences. Bio/Technology. 1992;10(4):413-417.

[10] Vandesompele J, et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002;3(7).

[11] Arya M, et al. Fundamentals of real-time PCR for diagnostic molecular biology. Adv Clin Chem. 2005;40:201-219.

[12] Mackay IM. Real-time PCR in the microbiology laboratory. Clin Microbiol Infect. 2004;10(3):190-212.

[13] Gibson UE, Heid CA, Williams PM. A novel method for real-time quantitative RT-PCR. Genome Res. 1996;6(10):995-1001.

[14] VanGuilder HD, Vrana KE, Freeman WM. Twenty-five years of quantitative PCR for gene expression analysis. Biotechniques. 2008;44(5):619-626.

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