Biofilms
Description
This page has 42 references
"Biofilms" are protective communities of microorganisms, such as bacteria or fungi, that adhere to surfaces and shield themselves within a slimy matrix. These structures often develop in moist environments like the gut, mouth, or wounds, making infections more persistent and harder to treat. Biofilms can trigger chronic inflammation and significantly reduce medication effectiveness. Supporting immune function, maintaining good hygiene, and using natural agents like cranberry or garlic may help disrupt biofilms and prevent stubborn, recurring infections.
Symptoms & Signs
Body System
Immune System
Causes
Microorganism Overgrowth, pH Imbalance, Diet
Things To Do
Dietary recommendations that can prevent the formation of biofilms and even break down existing ones include:
low-carbohydrate diet because carbohydrates support biomass formation.
diet rich in fiber.
the consumption of inulin-containing foods, such as asparagus, chicory root, banas, leeks, onions, dried garlic, etc., because they strengthen the gut mucosal barrier.
eating vegetables and low-glycemic fruits, such as berries.
The consumption of xylitol and stevia as sweeteners, both of which inhibit biofilm formation.
the consumption of apple cider vinegar [e.g., as a salad dressing], which helps break down existing biofilms.
eating anti-inflammatory foods and spices.
natural antibiotics, such as garlic, ginger, thyme, or oregano.
incorporating probiotic foods, such as kimchi, sauerkraut, kombucha [unsweetened], and pickled vegetables.
the consumption of foods that can inhibit cell signaling between microorganisms; these include turmeric [curcumin], garlic, apple cider vinegar, oregano oil, cinnamon, pomegranate, and vanilla beans.
Other recommendations include:
maintain proper hygiene. It is especially true for our oral cavity. Brush three times a day, floss regularly, use natural and safe mouthwash to maintain a healthy pH level, and visit your dental hygienist frequently.
If you have oral retainers, braces, implants, prosthetic medical devices, or other internal foreign materials in your body [such as pacemakers, etc.], learn about what you can do to prevent them from collecting microorganisms.
Familiarize yourself with available biofilm regulators; talk to your doctor.
Keep your GI tract healthy. It means frequent waste elimination and a balanced microbiome.
Things To Avoid
Dealing with biofilms can be tricky. These colonies of pathogenic microorganisms can form in various parts of the human body, including the oral cavity, the GI tract, and the nasal passages. In order to prevent their overgrowth, one should avoid:
high-carbohydrate diet.
inflammatory foods such as gluten, dairy, corn, soy, eggs, grains, and nightshades.
toxic foods such as sugar, alcohol, trans fats, food additives, and preservatives.
processed foods, processed meats, and pre-packaged meals and foods.
nutrient- and fiber-deficient diet.
Other things to avoid:
antibiotics. They are not an effective treatment for biofilms. They are unable to penetrate through the protective barrier the microorganisms have created for themselves.
the use of antibacterial agents in body care products and cleaning supplies, which, for the same reason as antibiotics, are ineffective in dealing with biofilms.
Smoking and smoke exposure trigger biofilm formation.
Supplements
Direct Support
(Most Helpful → Least Helpful)
These supplements directly affect biofilms by disrupting their structure, preventing bacterial adhesion, or enhancing immune responses that target embedded pathogens.
Serrapeptase: Serrapeptase disrupts biofilm membranes by breaking down their protective structure, exposing pathogenic bacteria or yeast, and making them more vulnerable to treatment.
N-Acetyl-L-Cysteine [NAC]: N-acetyl-L-cysteine (NAC) disrupts the structure of biofilms and prevents their formation, weakening bacterial colonies and making them more susceptible to treatment, while reducing bacterial load.
Glutathione: Glutathione disrupts biofilms by neutralizing oxidative stress within microbial colonies, weakening their structural defenses.
Nattokinase: Nattokinase breaks down established biofilms by degrading their structural components, helping to reduce bacterial infections and mitigate bacterial threats.
Probiotics: Probiotics compete with harmful bacteria, preventing biofilm formation and disrupting existing biofilms, supporting the body’s natural defenses and maintaining a healthy microbial balance.
Indirect Support
(Most Helpful → Least Helpful)
These supplements do not break down biofilms directly but may support detoxification, immune readiness, or microbial balance that influences biofilm resilience.
No replicated and convincing research studies are available, and there is no anecdotal evidence supporting the effectiveness of this method.
Natural Compounds
These natural compounds may support biofilm disruption or immune response through chelation, oxygenation, or antimicrobial activity.
Xylitol: Xylitol inhibits oral biofilm formation by disrupting bacterial adhesion, reducing dental plaque buildup and lowering the risk of tooth decay, supporting overall oral health.
Erythritol: Erythritol disrupts biofilm formation by preventing the development of the extracellular polymeric substance (EPS) that bacteria use to adhere to surfaces.
Sodium Bicarbonate: Baking soda is used anecdotally to alkalize surfaces and reduce microbial adhesion, with occasional application in biofilm-prone areas.
Hydrogen Peroxide: Hydrogen peroxide penetrates biofilms and generates reactive oxygen species that degrade bacterial structure and biofilm integrity.
Plant Extracts
Direct Support
(Most Helpful → Least Helpful)
These plant extracts directly target biofilms through natural compounds that degrade matrix structures, inhibit microbial cooperation, or enhance pathogen clearance.
Garlic Extract: Garlic, through its active compound allicin, disrupts biofilm formation by breaking down bacterial colonies and inhibiting their growth, helping reduce the risk of infections.
Curcumin: Curcumin inhibits biofilm formation by disrupting bacterial cell walls and signaling pathways involved in biofilm aggregation, making it useful for preventing biofilm-related infections.
Berberine: Berberine interferes with quorum sensing and bacterial adhesion, indirectly weakening biofilm formation and persistence.
Epigallocatechin Gallate [EGCG]: EGCG disrupts bacterial communication and limits adherence to surfaces, providing mild support against biofilm stability.
Cranberry Extract: Cranberry, rich in proanthocyanidins, prevents bacterial adherence and disrupts biofilms, particularly benefiting urinary tract health and preventing recurrent infections.
Indirect Support
(Most Helpful → Least Helpful)
These extracts do not act directly on biofilms but may support immune defense, reduce systemic inflammation, or help maintain microbial balance.
No replicated and convincing research studies are available, and there is no anecdotal evidence supporting the effectiveness of this method.
Alternative Treatments
These alternative therapies support the breakdown of biofilms, enhance internal detox processes, and promote a less hospitable environment for persistent microbes.
Ozone IV: Intravenous ozone introduces reactive oxygen molecules that disrupt biofilm structure and impair bacterial survival within protected colonies.
Ultrasound: Low-frequency ultrasound disrupts biofilms mechanically, loosening bacterial clusters and enhancing antimicrobial penetration.
Blue Light Therapy: Blue light therapy disrupts biofilm formation by directly targeting and destroying bacteria, preventing the development of biofilms and lowering the risk of infection.
Oil Pulling: Coconut oil used in oil pulling helps reduce dental plaque buildup by disrupting biofilm formation on tooth surfaces, thanks to its natural antimicrobial properties.
Functional Lab Tests You May Wish to Explore
Books Worth Considering for Deeper Insight
Currently, no high-quality books meeting our functional, integrative, and natural healing standards are available for this condition. As soon as trusted resources are published, we will review and feature them here.
Podcasts That Offer Valuable Perspectives
Currently, no high-quality books meeting our functional, integrative, and natural healing standards are available for this condition. As soon as trusted resources are published, we will review and feature them here.
Educational Videos To Learn From
How Key Nutrients Support the Body
Amino Acids: Amino acids serve as the building blocks of proteins, crucial for muscle growth and repair. Additionally, they play key roles in maintaining proper pH levels, storing nitrogen, synthesizing hormones, and facilitating enzymatic reactions.
Enzymes and Coenzymes: Enzymes catalyze biochemical reactions, while coenzymes assist in these processes by binding temporarily to enzymes. Together, they regulate digestion, energy production, immune responses, and various metabolic pathways.
Fruit Extracts: Rich in antioxidants, vitamins, and fiber, fruit extracts offer numerous health benefits, including anti-inflammatory, anti-cancer, and immune-boosting properties. They also support digestive health and contribute to overall well-being.
Herbal Extracts: Herbal extracts contain bioactive compounds with various medicinal properties, including antioxidant, anti-inflammatory, and antimicrobial effects. They have been used traditionally to support digestion, immunity, and overall health.
Probiotics: Probiotics are live microorganisms that confer health benefits when consumed. They support gut health, immune function, and oral hygiene by promoting the growth of beneficial bacteria and inhibiting the growth of harmful pathogens.
Essential Nutrients Explained
References
[1] Jadhav SB et al. Serratiopeptidase: Insights into the therapeutic applications. Biotechnology Reports. 2020, Vol 28, e00544.
[2] Zapotoczna M, McCarthy H, Rudkin JK, O'Gara JP, O'Neill E. An Essential Role for Coagulase in Staphylococcus aureus Biofilm Development Reveals New Therapeutic Possibilities for Device-Related Infections. J Infect Dis. 2015;212(12):1883-1893.
[3] Hogan S, Zapotoczna M, Stevens NT, Humphreys H, O'Gara JP, O'Neill E. Potential use of targeted enzymatic agents in the treatment of Staphylococcus aureus biofilm-related infections. J Hosp Infect. 2017;96(2):177-182.
[4] Iwamoto A, Nakamura T et al. The Japanese Fermented Food Natto Inhibits Sucrose-dependent Biofilm Formation by Cariogenic Streptococci. Food Science and Technology Research. 2018, 24. 129-137.
[5] Hogan S, O'Gara JP, O'Neill E. Novel Treatment of Staphylococcus aureus Device-Related Infections Using Fibrinolytic Agents. Antimicrob Agents Chemother. 2018;62(2):e02008-17.
[6] Blasi F, Page C, Rossolini GM, Pallecchi L, Matera MG, Rogliani P, Cazzola M. The effect of N-acetylcysteine on biofilms: Implications for the treatment of respiratory tract infections. Respiratory medicine. 2016 Aug 1;117:190-7.
[7] Dinicola S, De Grazia S, Carlomagno G, Pintucci JP. N-acetylcysteine as powerful molecule to destroy bacterial biofilms. A systematic review. European Review for Medical & Pharmacological Sciences. 2014 Oct 1;18(19).
[8] Quah SY, Wu S, Lui JN, Sum CP, Tan KS. N-acetylcysteine inhibits growth and eradicates biofilm of Enterococcus faecalis. Journal of endodontics. 2012 Jan 1;38(1):81-5.
[9] Schwendicke F, Korte F, Dörfer CE, Kneist S, Fawzy El-Sayed K, Paris S. Inhibition of Streptococcus mutan s Growth and Biofilm Formation by Probiotics in vitro. Caries Research. 2017 Mar 16;51(2):87-95.
[10] Meroni G, Panelli S, Zuccotti G, Bandi C, Drago L, Pistone D. Probiotics as therapeutic tools against pathogenic biofilms: have we found the perfect weapon?. Microbiology Research. 2021 Dec 6;12(4):916-37.
[11] Humphreys GJ, McBain AJ. Antagonistic effects of Streptococcus and Lactobacillus probiotics in pharyngeal biofilms. Letters in applied microbiology. 2019 Apr 1;68(4):303-12.
[12] Pérez‐Giraldo C, Cruz‐Villalón G, Sánchez‐Silos R, Martínez‐Rubio R, Blanco MT, Gómez‐García AC. In vitro activity of allicin against Staphylococcus epidermidis and influence of subinhibitory concentrations on biofilm formation. Journal of applied microbiology. 2003 Oct 1;95(4):709-11.
[13] Wu X, Santos RR, Fink‐Gremmels J. Analyzing the antibacterial effects of food ingredients: model experiments with allicin and garlic extracts on biofilm formation and viability of Staphylococcus epidermidis. Food science & nutrition. 2015 Mar;3(2):158-68.
[14] Lihua L, Jianhui W, Jialin Y, Yayin L, Guanxin L. Effects of allicin on the formation of Pseudomonas aeruginosa biofilm and the production of quorum-sensing controlled virulence factors. Polish Journal of Microbiology. 2013;62(3):243.
[15] Neelakantan P, Subbarao C, Sharma S, Subbarao CV, Garcia-Godoy F, Gutmann JL. Effectiveness of curcumin against Enterococcus faecalis biofilm. Acta Odontologica Scandinavica. 2013 Nov 1;71(6):1453-7.
[16] Vaughn AR, Haas KN, Burney W, Andersen E, Clark AK, Crawford R, Sivamani RK. Potential role of curcumin against biofilm‐producing organisms on the skin: A review. Phytotherapy research. 2017 Dec;31(12):1807-16.
[17] Duarte S, Gregoire S, Singh AP, Vorsa N, Schaich K, Bowen WH, Koo H. Inhibitory effects of cranberry polyphenols on formation and acidogenicity of Streptococcus mutans biofilms. FEMS microbiology letters. 2006 Apr 1;257(1):50-6.
[18] LaPlante KL, Sarkisian SA, Woodmansee S, Rowley DC, Seeram NP. Effects of cranberry extracts on growth and biofilm production of Escherichia coli and Staphylococcus species. Phytotherapy Research. 2012 Sep;26(9):1371-4.
[19] Girardot M, Guerineau A, Boudesocque L, Costa D, Bazinet L, Enguehard-Gueiffier C, Imbert C. Promising results of cranberry in the prevention of oral Candida biofilms. Pathogens and disease. 2014 Apr 1;70(3):432-9.
[20] Badet C, Furiga A, Thébaud N. Effect of xylitol on an in vitro model of oral biofilm. Oral health & preventive dentistry. 2008 Sep 1;6(4).
[21] Decker EM, Maier G, Axmann D, Brecx M, Von Ohle C. Effect of xylitol/chlorhexidine versus xylitol or chlorhexidine as single rinses on initial biofilm formation of cariogenic streptococci. Quintessence international. 2008 Jan 1;39(1).
[22] Brambilla E, Ionescu AC, Cazzaniga G, Ottobelli M, Samaranayake LP. Levorotatory carbohydrates and xylitol subdue Streptococcus mutans and Candida albicans adhesion and biofilm formation. Journal of basic microbiology. 2016 May;56(5):480-92.
[23] S Ferreira A, F Silva-Paes-Leme A, RB Raposo N, S da Silva S. By passing microbial resistance: xylitol controls microorganisms growth by means of its anti-adherence property. Current pharmaceutical biotechnology. 2015 Jan 1;16(1):35-42.
[24] Saran S, Mukherjee S, Dalal J, Saxena RK. High production of erythritol from Candida sorbosivorans SSE-24 and its inhibitory effect on biofilm formation of Streptococcus mutans. Bioresource technology. 2015 Dec 1;198:31-8.
[25] Janus MM, Volgenant CM, Brandt BW, Buijs MJ, Keijser BJ, Crielaard W, Zaura E, Krom BP. Effect of erythritol on microbial ecology of in vitro gingivitis biofilms. Journal of Oral Microbiology. 2017 Jan 1;9(1):1337477.
[26] Hashino E, Kuboniwa M, Alghamdi SA, Yamaguchi M, Yamamoto R, Cho H, Amano A. Erythritol alters microstructure and metabolomic profiles of biofilm composed of S treptococcus gordonii and P orphyromonas gingivalis. Molecular oral microbiology. 2013 Dec;28(6):435-51.
[27] Zope SA. Effect of coconut oil pulling on plaque-induced gingivitis: A prospective clinical study. International Journal of Green Pharmacy (IJGP). 2017;11(04).
[28] Asokan S, Emmadi P, Chamundeswari R. Effect of oil pulling on plaque induced gingivitis: A randomized, controlled, triple-blind study. Indian Journal of Dental Research. 2009 Jan 1;20(1):47-51.
[29] Hughes G, Webber MA. Novel approaches to the treatment of bacterial biofilm infections. British journal of pharmacology. 2017 Jul;174(14):2237-46.
[30] Halstead FD, Thwaite JE, Burt R, Laws TR, Raguse M, Moeller R et al. (2016). Antibacterial activity of blue light against nosocomial wound pathogens growing planktonically and as mature biofilms. Appl Environ Microbiol 82: 4006–4016.
[31] Klare W, Das T, Ibugo A, Buckle E, Manefield M, Manos J. Glutathione-disrupted biofilms of clinical Pseudomonas aeruginosa strains exhibit an enhanced antibiotic effect and a novel biofilm transcriptome. Antimicrobial agents and chemotherapy. 2016 Aug;60(8):4539-51.
[32] Das T, Paino D, Manoharan A, Farrell J, Whiteley G, Kriel FH, Glasbey T, Manos J. Conditions under which glutathione disrupts the biofilms and improves antibiotic efficacy of both ESKAPE and non-ESKAPE species. Frontiers in microbiology. 2019 Aug 30;10:2000.
[33] Zhang C, Li Z, Pan Q, Fan L, Pan T, Zhu F, Pan Q, Shan L, Zhao L. Berberine at sub-inhibitory concentration inhibits biofilm dispersal in Staphylococcus aureus. Microbiology. 2022 Sep 30;168(9):001243.
[34] Wang X, Yao X, Zhu ZA, Tang T, Dai K, Sadovskaya I, Flahaut S, Jabbouri S. Effect of berberine on Staphylococcus epidermidis biofilm formation. International journal of antimicrobial agents. 2009 Jul 1;34(1):60-6.
[35] Hengge R. Targeting bacterial biofilms by the green tea polyphenol EGCG. Molecules. 2019 Jun 29;24(13):2403.
[36] Lee P, Tan KS. Effects of Epigallocatechin gallate against Enterococcus faecalis biofilm and virulence. Archives of oral biology. 2015 Mar 1;60(3):393-9.
[37] Christensen BE, Trønnes HN, Vollan K, Smidsrød O, Bakke R. Biofilm removal by low concentrations of hydrogen peroxide. Biofouling. 1990 Aug 1;2(2):165-75.
[38] Presterl E, Suchomel M, Eder M, Reichmann S, Lassnigg A, Graninger W, Rotter M. Effects of alcohols, povidone-iodine and hydrogen peroxide on biofilms of Staphylococcus epidermidis. Journal of Antimicrobial Chemotherapy. 2007 Aug 1;60(2):417-20.
[39] Bialoszewski D, Pietruczuk-Padzik A, Kalicinska A, Bocian E, Czajkowska M, Bukowska B, Tyski S. Activity of ozonated water and ozone against Staphylococcus aureus and Pseudomonas aeruginosa biofilms. Medical science monitor: international medical journal of experimental and clinical research. 2011 Nov 1;17(11):BR339.
[40] Al‐Saadi H, Potapova I, Rochford ET, Moriarty TF, Messmer P. Ozonated saline shows activity against planktonic and biofilm growing Staphylococcus aureus in vitro: a potential irrigant for infected wounds. International wound journal. 2016 Oct;13(5):936-42.
[41] Zips A, Schaule G, Flemming HC. Ultrasound as a means of detaching biofilms. Biofouling. 1990 Nov 1;2(4):323-33.
[42] Dong Y, Xu Y, Li P, Wang C, Cao Y, Yu J. Antibiofilm effect of ultrasound combined with microbubbles against Staphylococcus epidermidis biofilm. International Journal of Medical Microbiology. 2017 Sep 1;307(6):321-8.